LOCUS       pLV-[hsa-miR-412]        9145 bp    DNA     circular      3-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7066..7926
                     /label=Amp(R)
     promoter        8127..8667
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5671..5905
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6480..6935)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5977..6107
                     /label=SV40 pA
     misc_feature    6108..6321
                     /label=SV40 ori
     misc_feature    4992..5588
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3610
                     /label=intron
     CDS             3657..4982
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3400
                     /label=hsa-mir-412 precursor
BASE COUNT     2149 a      2381 c      2497 g      2118 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca atgttgctcg 
     3121 gtgaacccct tttcggtatc aaattccacc agggaggccg tcttggaggc tggggcacct 
     3181 cggggaagga cgccggcatc agcaccattc tggggtacgg ggatggatgg tcgaccagtt 
     3241 ggaaagtaat tgtttctaat gtacttcacc tggtccacta gccgtccgta tccgctgcag 
     3301 cctgtggggc ctgcgggccg gggagccgat cgcgcttcag ctcagcgcct ttcctggtac 
     3361 ttgaagggag atcgaccgtg ttatattcgc tttattgact gctagctcga gcttttggag 
     3421 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg 
     3481 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct 
     3541 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct 
     3601 tccatttcag gtgtcgtgaa aactacccct ctagagtcga gctaccggtc gccaccatgg 
     3661 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 
     3721 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 
     3781 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 
     3841 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 
     3901 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 
     3961 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 
     4021 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 
     4081 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 
     4141 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 
     4201 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 
     4261 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 
     4321 ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggaccca ccggtcgcca 
     4381 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 
     4441 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 
     4501 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 
     4561 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 
     4621 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 
     4681 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 
     4741 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 
     4801 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 
     4861 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 
     4921 ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct 
     4981 gagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 
     5041 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 
     5101 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 
     5161 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 
     5221 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 
     5281 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 
     5341 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 
     5401 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 
     5461 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 
     5521 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 
     5581 cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat 
     5641 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 
     5701 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 
     5761 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 
     5821 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 
     5881 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 
     5941 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 
     6001 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
     6061 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 
     6121 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     6181 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
     6241 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag 
     6301 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
     6361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
     6421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 
     6481 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     6541 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     6601 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     6661 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     6721 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     6781 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     6841 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     6901 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
     6961 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     7021 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     7081 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     7141 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     7201 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     7261 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     7321 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     7381 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     7441 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     7501 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     7561 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     7621 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     7681 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     7741 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     7801 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     7861 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     7921 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     7981 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     8041 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     8101 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     8161 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     8221 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     8281 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     8341 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     8401 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     8461 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     8521 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     8581 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     8641 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     8701 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     8761 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     8821 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 
     8881 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     8941 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
     9001 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
     9061 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 
     9121 taaagggaac aaaagctgga gctgc 
//
